Transient early increase of S100A9 in bronchoalveolar and pleural fluids and of s100a8 / s100a9 transcripts in lungs.

<p>BALB/c mice were inoculated with 40 L3 <i>L</i>. <i>sigmodontis</i> either subcutaneously (SC) or intravenously (IV). Two hours (h2), six hours (h6), four days (d4) and 8 days (d8) post inoculation, mice were sacrificed. Bronchoalveolar and pleural lavages were performed then lungs were isolated and frozen. (A—D) Bronchoalveolar fluid (BAL) (A & B, respectively SC and IV infected mice; n = 6) and pleural fluid (PL) (C & D, respectively SC and IV infected mice; n = 10–12, pool of 3 independent experiments) were tested for S100A9 by ELISA. The results are expressed as mean ± SEM. One way ANOVA followed by a Bonferonni, ** = <i>p</i><0.01, * = <i>p</i><0.05 (difference between infected and naïve mice). nt: not tested. (E-F) A q-RTPCR was performed for (E) s100a8 and (F) s100a9 transcripts. Normalization was made with β-actin housekeeping gene by 2^-ΔΔCT method, n = 5–6 (pool of 3 independent experiments). The results are expressed as fold-change mean ± SEM; a two-way ANOVA followed by a Bonferonni was performed, *p<0.05 difference between IV-and SC- infected mice, ^##p<0.01, ^###p<0.001 difference between timepoints.