Murine S100A9 induced lung inflammation is RAGE-independent.

<p>Wild type C57Bl/6 or C57Bl/6 <i>ager</i>-/- mice were challenged intranasally with PBS, or adeno-null or adeno-murine S100A9, after 8 days, mice were sacrificed and BAL fluids or lung tissue were collected for analysis. (A) Cells were obtained from the BAL fluids using cytospins, stained with diff-quik and the total cell counts, neutrophil counts and macrophage counts were recorded. (B) mIFNγ and mIL-6 proteins levels found in BAL fluids of wild type and <i>ager</i>-/- mice. (C) Western blot analysis of murine S100A9 levels in BAL fluids. (D) H&E staining of paraffin fixed lung tissue (left panel), and pathology scores (right panel). Non-parametric Mann-Whitney test was used to determine statistical difference between two groups.