Time-dependent analysis of HRV72 propagation.

<p><b>(A)</b> Determination of viral RNA level changes in HRV72 infected A549 cells. <b>(B)</b> Determination of viral RNA level changes in supernatant of HRV72 infected A549 cells. QRT-PCR was performed using primers specific for both HRV72 and Psmb2 at indicated time points and sample types. The amount of the HRV72 RNA was normalized to the amount of Psm2 mRNA. The relative abundance was calculated by standard curve method. Values on the y-axis are presented after log₂ transformation. The induction of viral RNA relative to mock-infected cells is shown on a log₂ scale. Each error bar represents standard deviation of mean viral RNA level from three independent experiments. Fold changes are indicated for each time compared with 1.5 hpi in Fig A and PBS in Fig B. *: a significant increase compared with 1.5 hpi (p<0.05, by post hoc comparison using Tukey test); hpi: hours post infection.