An analysis of various H-I loop mutant viruses.

<p><b>(A)</b> A sequencing chromatogram of the mutated H-I loop region. <b>(B)</b> Viral titers in the supernatant were measured at 48 hpi after the transfection of EV71 mutant viral RNA. Each column represents the average of two replicates. Error bars represent the SEM * 0.01(C) or with <b>(D)</b> inhibitor HL051001P2 treatment. The EV71 infection was quantified by using qRT-PCR to detect the EV71 RNA in cells at different times post infection, and the mRNA level of GAPDH was used as an internal control.