Rarefaction comparing expected OTU richness of pooled samples (grey lines) and individual replicates (black lines).

<p>Only replicates with more than 15,000 reads were included from the samples Fine Sand (A; n = 5), Coarse Sand (B; n = 7) and Clay (C, n = 5). Panel D shows expected richness in pooled samples compared to mean expected replicate richness. Error bars represent standard error, for pooled samples calculated as described in Heck et al [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179443#pone.0179443.ref047" target="_blank">47</a>].