Relative abundance of sequences used to generate bacterial mock communities.

<p>A: mock1, B: mock 2, C: mock 3. Dashed line: theoretical relative abundance. The experimental mean relative abundance for small libraries (green circles) and large libraries (red circles) are shown and error bars indicate standard deviations for triplicate samples. The grey box indicates a sequence that was not detected in any community; the black box indicates an OTU that consisted of two sequences at a similarity cutoff of 3%.