Numbers of NeuN-immunoreactive neurons in the thalamic area and cortex of WT, homozygous <i>Slc19a3</i> KO and KI mice.

<p>The numbers of NeuN-immunoreactive neurons of homozygous KO (A) and KI (D) mice in the submedial nucleus of the thalamus (SMT) (0.13 mm²); homozygous KO (B) and KI (E) mice in the ventral anterior-lateral complex of the thalamus (VAL) (0.2 mm²); and homozygous KO (C) and KI (F) mice in the cortex (0.2 mm²). Mean numbers of three experiments are shown. One-way ANOVA, two-way ANOVA, and the Steel–Dwass test were used for statistical comparisons. Asterisks (*) indicate that the numbers of NeuN-immunoreactive neurons of KO and KI mice fed with thiamine-restricted diets were significantly different from those of WT mice (first bar of each graph) at p < 0.05.