Recovery of homozygous <i>Slc19a3</i> KO mice with a high-thiamine diet after thiamine restriction.

<p>Homozygous KO mice were fed a thiamine-restricted diet (thiamine 0.60 mg/100 g food) for 2 days and then changed to a high-thiamine diet (thiamine 8.50 mg/100 g food) for 28 days. A. Low magnification of the NeuN-immunoreactive neurons in the thalamic area in a recovered homozygous KO mouse. B. High magnification of the inset of panel A. The submedial nucleus of the thalamus (SMT) and ventral anterior-lateral complex of the thalamus (VAL) are shown. C, GFAP immunostaining of the serial sections of B, respectively. Scale bar, 500 μm. The mean numbers of NeuN-immunoreactive neurons in the thalamic area and cortex of 3 homozygous KO mice are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0180279#pone.0180279.g004" target="_blank">Fig 4A–4C</a>.