Identification of the chi-miR-4110 target gene.

<p><i>Smad2</i> gene was predicted as an important target. (A) chi-miR-4110 binds at positions 1706–1727 of <i>Smad2</i> mRNA. (B) The wild-type psiCHECK-2–Smad2–3′UTR (WT) vector and mutant psiCHECK-2–Smad2–3′UTR (Mutant) vector, which contained seven continuous mutant nucleotides, were constructed by a dual luciferase reporter system. (C) Smad2–3′UTR–WT and Mutant sequences. Mutated bases are in italics. (D) The vectors were co-transfected with either chi-miR-4110 mimics or mimics negative control (NC). After 36 h of transfection, GCs were collected and subjected to dual-luciferase assay. Relative luciferase activity was determined by the ratio of firefly to <i>Renilla</i> luciferase activity. Data are presented as mean activities ± standard deviation. Different small letters represent a significant difference at the 5% level.