Additional file 1: Figure S1. of Distinct effects of EGFR inhibitors on epithelial- and mesenchymal-like esophageal squamous cell carcinoma cells

Effects of EGFR inhibition or activation on immortalized-human esophageal epithelial cells. EPC2-hTERT, an immortalized-human esophageal epithelial cell line, was treated with erlotinib (1 μM) or cetuximab (100 μg/mL). (A) Cytokeratin13 (CK13) mRNA expression levels in EPC2-hTERT cells treated with or without erlotinib or cetuximab for 72 h determined by QPCR. The gene for β-actin served as an internal control. (**p < 0.01 vs. vehicle control; n = 3). (B) CK13 mRNA expression levels in EPC2-hTERT cells treated with recombinant EGF (rEGF) for 48 h determined by QPCR. The gene for β-actin served as an internal control. (**p < 0.01 vs. vehicle control; n = 3). (JPEG 130 kb