Additional file 4: Figure S4. PCR screening results for recombinant B. subtilis and B. licheniformis harboring the 15 pHT01-kIspS plasmid. Lane 1: Negative control. Lane 2: PCR results for pHT01-kIspS in B. 16 licheniformis. Lane 3: positive control from pHT01-kIspS. Lanes 4, 5 & 6: PCR results for pHT01-17 kIspS in B. subtilis. Marker: 2 log DNA ladder (1.0â10.0 kb) NEB catalogue #N04695
Additional file 1: Figure S1. PCR analysis of the Mag II-CB (a) and CB (b) gene in wild-type and tra...
Additional file 1: Figure S1. (A) Confirmation of HindIII insertion near pikRII with apr R check pri...
Additional file 1: Table S1. Primers used in this study. Fig S1. The mutagenesis selection process. ...
Additional file 3: Figure S3. Digestion of pHT01 plasmid and the amplified kIspS fragment with BamHI...
Abstract Isoprene represents a key building block for the production of valuable materials such as l...
Additional file 1: Figure S1. Diagram of the locations for antibiotic substance gene clusters in B. ...
Additional file 3: Figure S3. Confirmation of the recombinant strains of B. licheniformis by PCR amp...
Additional file 1. Table S1 . Constructed plasmids and strains in this study; Table S2. Primers used...
PCR amplification of specific gyr A gene fragment (1027 bp) in B. subtilis . Lane M: 100 bp plus DNA...
Additional file 5: Figure S5. Analysis of the recombinant plasmid pET-gdh (A), and confirmation of t...
We have discovered that microorganisms produce and emit the hydrocarbon isoprene (2-methyl-1,3-butad...
The yvmA-yvmC-cypX locus from B. subtilis and B. licheniformis ATCC 14580. (A) For each gene product...
Additional file 2. Figure S1: Confirmation experiments for deletion mutants. A PCR fragments from ge...
Additional file 1: Text S1. Construction of integration vectors; mCherry reporter gene expression in...
Additional file 1: Table S1. The sequences of nat1 and ble genes used in the current study. Table S2...
Additional file 1: Figure S1. PCR analysis of the Mag II-CB (a) and CB (b) gene in wild-type and tra...
Additional file 1: Figure S1. (A) Confirmation of HindIII insertion near pikRII with apr R check pri...
Additional file 1: Table S1. Primers used in this study. Fig S1. The mutagenesis selection process. ...
Additional file 3: Figure S3. Digestion of pHT01 plasmid and the amplified kIspS fragment with BamHI...
Abstract Isoprene represents a key building block for the production of valuable materials such as l...
Additional file 1: Figure S1. Diagram of the locations for antibiotic substance gene clusters in B. ...
Additional file 3: Figure S3. Confirmation of the recombinant strains of B. licheniformis by PCR amp...
Additional file 1. Table S1 . Constructed plasmids and strains in this study; Table S2. Primers used...
PCR amplification of specific gyr A gene fragment (1027 bp) in B. subtilis . Lane M: 100 bp plus DNA...
Additional file 5: Figure S5. Analysis of the recombinant plasmid pET-gdh (A), and confirmation of t...
We have discovered that microorganisms produce and emit the hydrocarbon isoprene (2-methyl-1,3-butad...
The yvmA-yvmC-cypX locus from B. subtilis and B. licheniformis ATCC 14580. (A) For each gene product...
Additional file 2. Figure S1: Confirmation experiments for deletion mutants. A PCR fragments from ge...
Additional file 1: Text S1. Construction of integration vectors; mCherry reporter gene expression in...
Additional file 1: Table S1. The sequences of nat1 and ble genes used in the current study. Table S2...
Additional file 1: Figure S1. PCR analysis of the Mag II-CB (a) and CB (b) gene in wild-type and tra...
Additional file 1: Figure S1. (A) Confirmation of HindIII insertion near pikRII with apr R check pri...
Additional file 1: Table S1. Primers used in this study. Fig S1. The mutagenesis selection process. ...