MOESM1 of Improvement of isoprene production in Escherichia coli by rational optimization of RBSs and key enzymes screening

Additional file 1. Table S1 . Constructed plasmids and strains in this study; Table S2. Primers used in this study; Fig. S1. OD600 of strains constructed in this study. a. OD600 of strain engineered with IDI modification. b. OD600 of strain engineered with MK modification. c. OD600 of strain engineered with IspS modification. d. OD600 ofstrain engineered through combination of the three enzymes, IDIsa, MKmm and IspSib. e. OD600 of strain with modification of RBS sequence of MvaE. f. OD600of strain with modification of RBS sequence of ERG19. The black column indicated the OD600 of original strain. The white column indicated that OD600 of strain with only enzyme substitution. The gray column indicated the OD600of strain with RBS sequence optimization. The experiment was conducted in triplicate. Bar represents meanÂąs.d.; Fig. S2. Yields of strains constructed in this study. a. Yields of strain engineered with IDI modification. b. Yields of strain engineered with MK modification. c. Yields of strain engineered with IspS modification. d. Yields of strain engineered through combination of the three enzymes, IDIsa, MKmm and IspSib. e. Yields of strain with modification of RBS sequence of MvaE. f. Yields of strain with modification of RBS sequence of ERG19. The black column indicated the isoprene yields of original strain. The white column indicated that isoprene yields of strain with only enzyme substitution. The gray column indicated the isoprene yields of strain with RBS sequence optimization. The experiment was conducted in triplicate. Bar represents meanÂąs.d