MOESM1 of Expression of a recombinant hybrid antimicrobial peptide magainin II-cecropin B in the mycelium of the medicinal fungus Cordyceps militaris and its validation in mice

Additional file 1: Figure S1. PCR analysis of the Mag II-CB (a) and CB (b) gene in wild-type and transformed C. militaris. The PCR products were analyzed on a 1.0% (w/v) agarose gel. Lane 1: bank control (ddH2O). Lane 2: negative control (WT C. militaris). Lane 3: positive control (plasmid pCB130-Mag II-CB or pCB130-CB). Lanes 4–15 (a): PCR products generated from Mag II-CB transformants. Lanes 4–18 (b): PCR products generated from CB transformants. M, 2000-bp DNA ladder. Table S1. Determining the minimal lethal dose (MLD) for E. coli (ATCC 25922) in mice. Table S2. Determining the concentration of the recombinant peptide needed for antibacterial protection