<p><b>A.</b> Size exclusion chromatography (SEC) on HCMV PC purified from cell supernatant with a 12E2 IgG resin. <b>B.</b> SDS-PAGE analysis of PC 12E2 affinity purification eluate and SEC fractions collected in (A) under reducing conditions. kDa = kilo Dalton; FT = flow through, T = test eluate, P = final prep eluate. <b>C.</b> ELISA binding of PC- and gH-specific NAb to the purified PC. NAb targeting different epitopes of the UL128/130/131A subunits (1B2, 54E11, 21F6, 13B5) or gH (18F10, 62–11, 62–100, AP86) were tested in saturating amounts to bind the purified PC protein via ELISA. Error bars represent difference in binding of three independent experiments. <b>D.</b> Inhibition of EC entry by purified PC protein. Serial dilutions of pu...
<p>ELISA binding of Fab purified antibody fragments. <b>A.</b> Binding of anti-PC clones p2–20, p2–3...
Binding affinity of obtained anti-E2 antibodies to E2 proteins from (A) TH and (B) J6CF was analyzed...
<p>PC interacts with NS5A derived from both genotype 1b and 2a. Either Huh7.5 cells (A) or HEK293T c...
<p>(A) Anti-gB antibody detects full length gB (160 kD) and furin cleaved gB (55 kD doublets) in elu...
<p>(A) One hundred microliters of sera from five HCV-positive patients were incubated with either th...
<p>A vector encoding wild-type E2<sub>660</sub> was transfected into CHO cells grown in defined medi...
<p>(A) Pentameric gH/gL/pUL128-131 complex, (B) gH/gL and (C) gB complexes were subjected to SDS-PAG...
<p>(A) Human fibroblasts were infected with HCMV BADrUL131 for 3 days then cells were pelleted and c...
<p>(A) Undiluted NHS were incubated at room temperature for 1, 3, and 6 h. Purified C3 protein (50 µ...
<p>ELISA plates were coated with HCV proteins and the relative binding of the different NTFs was mon...
<p>ARPE-19 or MRC-5 cells were transduced with Ad vectors expressing HCMV gB, gH/gL, both gB and gH/...
Recombinant autologous 212 1b (square symbol) and H77C (circle symbol) E1E2 lysates were either left...
<div><p>Human Cytomegalovirus (HCMV) is a major cause of morbidity and mortality in transplant patie...
<p>(<b>A</b>) Cytotect was coated to a plate in binding buffer (0.1 M Na<sub>2</sub>HPO<sub>4</sub> ...
<p>(A) ARPE-19 cells were transduced with Ad vectors expressing Ad-tet-trans alone (tet, as a negati...
<p>ELISA binding of Fab purified antibody fragments. <b>A.</b> Binding of anti-PC clones p2–20, p2–3...
Binding affinity of obtained anti-E2 antibodies to E2 proteins from (A) TH and (B) J6CF was analyzed...
<p>PC interacts with NS5A derived from both genotype 1b and 2a. Either Huh7.5 cells (A) or HEK293T c...
<p>(A) Anti-gB antibody detects full length gB (160 kD) and furin cleaved gB (55 kD doublets) in elu...
<p>(A) One hundred microliters of sera from five HCV-positive patients were incubated with either th...
<p>A vector encoding wild-type E2<sub>660</sub> was transfected into CHO cells grown in defined medi...
<p>(A) Pentameric gH/gL/pUL128-131 complex, (B) gH/gL and (C) gB complexes were subjected to SDS-PAG...
<p>(A) Human fibroblasts were infected with HCMV BADrUL131 for 3 days then cells were pelleted and c...
<p>(A) Undiluted NHS were incubated at room temperature for 1, 3, and 6 h. Purified C3 protein (50 µ...
<p>ELISA plates were coated with HCV proteins and the relative binding of the different NTFs was mon...
<p>ARPE-19 or MRC-5 cells were transduced with Ad vectors expressing HCMV gB, gH/gL, both gB and gH/...
Recombinant autologous 212 1b (square symbol) and H77C (circle symbol) E1E2 lysates were either left...
<div><p>Human Cytomegalovirus (HCMV) is a major cause of morbidity and mortality in transplant patie...
<p>(<b>A</b>) Cytotect was coated to a plate in binding buffer (0.1 M Na<sub>2</sub>HPO<sub>4</sub> ...
<p>(A) ARPE-19 cells were transduced with Ad vectors expressing Ad-tet-trans alone (tet, as a negati...
<p>ELISA binding of Fab purified antibody fragments. <b>A.</b> Binding of anti-PC clones p2–20, p2–3...
Binding affinity of obtained anti-E2 antibodies to E2 proteins from (A) TH and (B) J6CF was analyzed...
<p>PC interacts with NS5A derived from both genotype 1b and 2a. Either Huh7.5 cells (A) or HEK293T c...