HCMV gB and gH, but not ICP8, obtained from infected cell lysates binds to a THY-1 protein column.

<p>(A) Anti-gB antibody detects full length gB (160 kD) and furin cleaved gB (55 kD doublets) in eluates of HCMV-infected cell lysates from THY-1 protein columns with either of two lysis buffers, but not from the VZV gE protein column. The very dark 115 kDa band seen in the THY-1 and gEt bands are background bands likely due to cross-reactivity of the anti-gB or secondary antibody with protein from the His column. (B) Anti-ICP8 antibody detects a 135 kD protein band in lysate from MRC-5 cells infected with HCMV AD169, but not from eluates of lysates applied to THY-1 or VZV gE protein columns. Infected cell lysates were prepared using lysis buffer 1 (PBS with 0.1% NP-40) or lysis buffer 2 (25 mM Tris, 15 mM NaCl and 0.1% NP-40). (C) Anti-gH antibody detects gH (92 kD) in eluates of HCMV-infected cell lysates from THY-1 protein columns with two different lysis buffers. gH was not detected in eluate from a control varicella-zoster gE column.