SDS-PAGE and Western blot (WB) analysis of soluble HCMV glycoprotein complexes.

<p>(A) Pentameric gH/gL/pUL128-131 complex, (B) gH/gL and (C) gB complexes were subjected to SDS-PAGE and Western blotting. Samples were stained with Coomassie blue (left panels) or probed with specific antibodies after WB (right panels). (B) A band of about 60 kDa on gL blot is apparently a co-purified contaminant recognized by anti-gL polyclonal serum raised in rabbits. (C) Soluble gB exists in full-length and truncated forms that associate in dimeric complex, as described in Carlson et al. (32). N-terminus of truncated gB form is not detected since gB was his-tagged on the C-terminus and the blot was probed with anti-histag antibody.