Phylogenetic relationship of the Hsp110/HSPH protein family from <i>G</i>. <i>m</i>. <i>morsitans</i> (Gmm), <i>D</i>. <i>melanogaster</i> (Dmel), <i>S</i>. <i>calcitrans</i> (Scal), and <i>H</i>. <i>sapiens</i> (Hs).

<p>Multiple sequence alignment of the full-length amino acid sequences of the Hsp110/HSPH gene families in humans, tsetse flies, fruit flies, and stable flies. The multiple sequence alignment (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183858#pone.0183858.s002" target="_blank">S2 Fig</a>) was performed using the in-built ClustalW program [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183858#pone.0183858.ref043" target="_blank">43</a>] with default parameters on the MEGA7 software [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183858#pone.0183858.ref044" target="_blank">44</a>]. The phylogenetic tree was constructed by MEGA 7 using the Maximum-likelihood method based on the Le Gascuel (LG) matrix-based model of amino acid substitution. A discrete Gamma distribution was used to model evolutionarily rate differences among sites (2 categories (+G, parameter = 1.3525). The alignment gaps were excluded from the analysis, and the number of amino acid sites used to construct the tree numbered 544. Bootstrap analysis was computed with 1000 replicates. Accession numbers of the sequences used: <i>E</i>. <i>coli</i>: HscC (NP_415183.1). <i>S</i>. <i>calcitrans</i>: Hsp110 (SCAU005995); Grp170 (SCAU010922). D. melanogaster: Hsp110 (NP_648687.1); Grp170 (NP_569995.1). <i>H</i>. <i>sapiens</i>: HSPH1 (NP_006635.2); HSPH2 (NP_002145.3); HSPH3 (NP_055093.2); HSPH4 (NP_006380.1). Accession numbers for the <i>G</i>. <i>m</i>. <i>morsitans</i> Hsp110 sequences can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183858#pone.0183858.t001" target="_blank">Table 1</a>. The subcellular localisation for Hsp110s is indicated by a bracket on the right. CYT: cytosolic; ER: endoplasmic reticulum.