Bioinformatic comparison and phylogenetic analysis of <i>Hikaru genki</i> (<i>Hig</i>) in insects.

<p>(A) Percentage of amino acid identity between AaHig and <i>Drosophila melanogaster</i> Hig (DmHig). (B) Schematic representation of AaHig and DmHig. The functional modules were predicted using the SMART (<a href="http://smart.embl-heidelberg.de/smart/set_mode.cgi?GENOMIC=1" target="_blank">http://smart.embl-heidelberg.de/smart/set_mode.cgi?GENOMIC=1</a>) and Pfam (<a href="http://pfam.sanger.ac.uk/" target="_blank">http://pfam.sanger.ac.uk/</a>) websites. (C) Unrooted phylogenetic tree of insect Higs. The tree was constructed using the neighbour-joining (NJ) method based on the alignment of insect Hig protein sequences. The bootstrap values of 5000 replicates are indicated on the branch nodes. <i>Drosophila</i> spp. (<i>Dr</i>.); <i>Ceratitis</i> spp. (<i>Ct</i>.); <i>Musca</i> spp. (<i>Mu</i>); <i>Anopheles</i> spp. (<i>An</i>.); <i>Aedes</i> spp. (<i>Ae</i>.); <i>Culex</i> spp. (<i>Cx</i>); <i>Tribolium</i> spp. (<i>Tr</i>.); <i>Dendroctonus</i> spp. (<i>De</i>.); <i>Nasonia</i> spp. (<i>Na</i>.); <i>Cerapachys</i> spp. (<i>Cp</i>.); <i>Camponotus</i> spp. (<i>Ca</i>.); <i>Acromyrmex</i> spp. (<i>Ac</i>.); <i>Solenopsis</i> spp. (<i>So</i>.); <i>Megachile</i> spp. (<i>Me</i>.); <i>Bombus spp</i>. (<i>Bu</i>.); <i>Apis spp</i>. (<i>Ap</i>.); <i>Danaus spp</i>. (<i>Da</i>.); <i>Bombyx spp</i>. (<i>By</i>.).