Inhibition of miR-30b enhances endothelial capillary morphogenesis.

<p>(A) HUVECs were transfected with either control miRNA inhibitor or miR-30b specific inhibitor for 48 hours. Data presented is the mean ± SEM (n = 2) normalized to the expression of miR-30b in control inhibitor transfected cells. * <i>P</i> < 0.05, ** <i>P</i> < 0.01, *** <i>P</i> < 0.001 as determined by unpaired Student’s <i>t</i>-test. (B) Morphology of HUVECs is unaffected by reduction of miR-30b using specific inhibitor (50 nM). (C) HUVECs transfected with miR-30b inhibitor (50 nM) were assessed for cell viability by trypan blue exclusion at the times indicated. Data represents the mean ± SEM (n = 2). (D) Migration of HUVECs depleted of miR-30b by specific inhibitor was assessed by scratch wound assay. Data represents the mean ± SEM (n = 2) for distance migrated after 24 hours and normalized to initial wound size. (E) Representative images of HUVEC capillary morphogenesis after 24 hours on growth factor reduced BME. Cells were transfected with control or miR-30b specific inhibitor and the total number of capillary-like cord structures and number of loops formed was assessed from duplicate wells. (F) Data represents the mean ± SEM for cells transfected with 50 nM (n = 3) or 0.1 nM (n = 2) miR-30b inhibitor. Capillary-like cord formation is significantly enhanced in cells with reduced levels of miR-30b. * <i>P</i> < 0.05, ** <i>P</i> < 0.01 as determined by unpaired Student’s <i>t</i>-test.