VEGF reduces miR-30b expression in HUVECs.

<p>(A) RNA was isolated from HUVEC treated with 50ng/ml of VEGF for 24h and used to profile miRNA using Affymetrix gene expression arrays. Bars represent the fold changed over unstimulated control HUVEC for some of the top targets (n = 1). (B) Cells were serum starved overnight in MCDB 131 with 0.5% FBS and stimulated with VEGF (50 ng/ml) for 24 hours. Total RNA was extracted and subjected to qRT-PCR to assess miR-30b expression. Levels of miR-30b are presented as the mean ± SEM relative to the expression of the endogenous controls miR-103 (n = 6) and RNU24 (n = 3). A statistically significant decrease in miR-30b expression is observed following VEGF stimulation. ** <i>P</i> = 0.0024 vs. miR-103; * <i>P</i> = 0.014 vs. RNU24 as determined by unpaired Student’s <i>t</i>-test.