Inhibition experiments with PP PLA₂.

<p>(A) Dose-response curve of PP PLA₂ inhibitory action on the ACh-evoked (25 μM ACh) ionic currents mediated by human α9α10 nAChR heterologously expressed in <i>Xenopus</i> oocytes. (B) Inhibition by PP PLA₂ of the initial rate of specific [¹²⁵I]-α-Bgt binding to <i>T</i>. <i>californica</i> and human hα7 nAChRs expessed in GH₄C₁ cells. Only 30% of binding sites in hα7 nAChRs could be protected from [¹²⁵I]-α-Bgt binding. (C) Inhibition by PP PLA₂ of specific [¹²⁵I]-α-Bgt binding to ECD of human α9 nAChR. IC₅₀ 5.5 μM.