RSV cellular immune responses in AGMs following infection.

<p>RSV-specific cellular immune responses in AGMs at 28 days following infection were determined by multiparameter flow cytometry. Cytokine secretions including IFN-γ, IL-2, and TNF-α of CD4⁺ (A) and CD8⁺ (B) T cells in PBMC specific to various RSV antigen stimulations are depicted (line represents mean). (C). Cytokine secretions of CD8⁺ T cells in lung in response to RSV antigens F and N (line represents mean) were depicted. (D). Comparison of RSV F- and N-specific CD8⁺ T cell responses (represented by IFN-γ-secreting CD8⁺ T cells) in PBMC and in lung (N = 8) with p value for paired t test was depicted. (E). Polyfunctionality of RSV-specific CD8⁺ T cell responses in PBMC and BAL (N = 8) were analyzed by Boolean gating using FlowJo software and graphed using SPICE software. The percentage of RSV N-specific CD8⁺ T cells in PBMC and lung secreting one cytokine, 2 cytokines, or 3 cytokines at the same time are depicted respectively in the pie charts. The absolute magnitude of each population secreting individual cytokines, or combinations of two or three cytokines, are depicted in the scattered dot plots below the pie charts (line represent mean). *, p<0.05, one-way ANNOVA compared to the other populations.