Airway CD8 T cells secrete excessive amounts of IFN-γ upon RSV infection in M2₈₂-immunized mice.

<p>IFN-γ protein amount was determined in the (A) lung and (B) serum at 0, 2, and 4 days following RSV challenge of immunized mice. (C) DC-LM-immunized mice were treated with 250 μg BFA 6 hours prior to tissue collection. In vivo IFN-γ production was assessed for major leukocyte populations in the lung 2 days following either mock or RSV infection. Frequency of IFN-γ-producing CD8 T cells based upon (D) CD11a expression and (E) M2₈₂ epitope specificity 2 days after either mock or RSV infection. (F) Frequency of IFN-γ⁺ CD8 T cells was assessed in the lung, BAL, mLN, spleen, and PBL at day 2 p.i. Data are represented as mean ± SEM of two independent experiments (<i>n</i> = 6 for control and <i>n</i> = 8 for M2₈₂ in (A, B); <i>n</i> = 8 for (C-F)). Groups were compared using Student’s <i>t</i> test for (A-E) and one-way ANOVA in (F), * <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001.