LPS stimulates ERK1/2 and PLA₂- phosphorylation via TLR4 and Akt.

<p>Washed human platelets were incubated for 3 minutes with LPS RS (1μg/mL) or Akt inhibitor IV (5μM) before activation with U46619 (0.25μM) in the presence or absence of vehicle or LPS from <i>E</i>. <i>coli</i> (1μg/mL) and were analysed by immunoblotting using antiphospho- ERK1/2 (A and B) and antiphospho-PLA₂ (A and C) antibodies. Total levels of 14-3-3ζ were measured on each sample as a loading control. Cumulative data represent mean values ± SEM (n = 4). (Anova-Bonferroni test, * P≤ 0.05; ** P≤ 0.01).