Demonstration of complete CST injury.

<p>Transverse section of the cervical spinal cord rostral to the dorsal column crush injury immunolabelled for PKCγ to show intact CST axons (A). The arrow indicates the location of the CST in the dorsal columns. Transverse section taken caudal to the site of injury showing the absence of corticospinal axons in the dorsal columns (B). Spinal cord lesion can be visualised in thionin-eosin stained spinal cords of animals receiving OEC-GFP (C) and OEC-ChABC (D). No significant differences in the lesion size were detected between the two groups (n = 3). Scale bar 500μm.