KANDUNGAN RESERPIN KULTUR KALUS PULE PANDAK (Rauvolfia verticillata (Lour.) Baillon) SETELAH DIELISITASI DENGAN CENDAWAN Pythium sp.

ABSTRACT Ninik Puji Astuti. 2007. RESERPINE CONTENT OF Rauvolfia verticillata (Lour.) Baillon CALLUS CULTURE ELICITED BY ELICITOR DERIVED FROM Pythium sp. Biology. Mathematics and Natural Science Faculty. Sebelas Maret University. A research to study the effect of elicitor derived from Pythium sp. on reserpine content of Rauvolfia verticillata (Lour.) Baillon callus culture has been conducted on June-December 2006. Callus was induced from R. verticillata’s leaf segment, and grew optimally on Murashige and Skoog’s medium with the addition of 2 mg/L NAA and 2 mg/L kinetin. Callus was elicited with elicitor derived from autoclaved Pythium sp. on the 30th day. The concentration of elicitor were: 0.0, 0.5, 1.0, 1.5, and 2.0 mg DW/ml. The harvesting time of the elicited callus were at 0, 18, 36, and 72 hours. Morphology of the callus (texture and color) were observed descriptively. Dry weight of callus and reserpine content were analyzed statistically using General Linear Model Univariat (GLM Univariat) and followed by Duncan’s Multiply Ring Test (DMRT) at 95% of confidence. This research showed that concentration of elicitor and harvesting time influenced the dry weight and reserpine content of R. verticillata callus culture significantly. Two mg DW/ml elicitor with harvesting time at 72 hours produced the lowest dry weight of callus that was 0.039 g, but produced the highest reserpine content that was 481.900 mg/g DW of callus or increased 103.849% compared to the control