Pengaruh manipulasi media terhadap kandungan alkaloid ninkristina kalus daun Cathasranthus roseus L Gdon

The effect of the manipulation of the media on the vincristine alkaloid content in the callus of Cantaranthus roseus (L) G.Don were studied. This work was done as an effort for gaining the the vincristine alkaloid through tissue culture which was expected to obtain a large amount of the alkaloid. Tissue culture of C. roseus was initiated from leaf explants on growth medium (MS supplmtented with I mg/l 2,4-D and 1 mg/l BAp). After seven weeks incubation, with only once subculturing on the some mediun, the proliferating calli were subcultured on a production medium (Ms supplemented with 1 mb/l IAA and 1 mg.l BAP) which were containing different additional concentration of sucrose, BAP, tryptophan, and concentration of 50% basic medium of MS from the standard. Eleen week=old calli were harvested from each treatment and dried for chemical analysed by thin layer chromatography on silica gel GF 254 using chloroform-aceton-thiethylamine as mobile phase. Rf value and UV spectra were used to identify vincristine, and concentration of vincristine alkaloid was determine by preparative thin layer chromatography with methanol solvent and measured by UN-Vis spectrophotometer at 233 nm. The maximum content of vincristine alkaloid was obtained from the callus, which was grown on the MS standard with addition og 50 g/l sucrose or 4 mg/l BAP or also 50% of the MS standard medium. Tryptophan addition as precursor could not induce the alkaloid vincristine forming