Protocols in Molecular Biology, John Wiley & Sons,

plasmid vector and the presence of the full gene was confirmed by partial sequencing from both ends (Figure 2). Sequencing of the full gene is in progress. Figure 2 Cloning of acyl-ACP thioesterase Lanes A and B. phage DNA from 15 I2 digested with BomHl and BamHI+Sall, respectively Lanes D and E (lanes A and B respectively), Southern hybridization of digested phage DNA with thioesterase probe Lanes F and G (two different clones) BornHl fragment ( ~ 4 0 kb) cloned into pBluescript SK-, and lanes I and J respectively show Southern hybridization of these clones with thioesterase probe Lanes C and H marker DNA (LDNA. digested with EcoRl and Hindlll