Confirmation of pHel plasmid transfer into transconjugants.

<p>(A) Isolation of plasmid DNA from transconjugants after co-cultivation of <i>H. pylori</i> P12 donor and recipient strains. Isolation of plasmid DNA after co-cultivation of <i>H. pylori</i> P12 pHel12::<i>cat</i> (donor) with <i>H. pylori</i> P12[<i>moeB::aphA-3</i>]. Presence of the donor plasmid pHel12::<i>cat</i> in the recipient strain (red arrow, lane 3,4) and of the recipient plasmid pHel12 (black arrow, lanes 3 and 4) is shown. Lane 1: 1 kb ladder, lane 2: pHel12, lane 3: transconjugant 1, lane 4: transconjugant 2. (B) Scheme explaining the PCR for the specific detection of donor- and recipient plasmid in transconjugants using oligos SR57 and SR58. (C) PCR fragments obtained with primers SR57/SR58 for the donor plasmid (red arrow, 1.3 kb) and for the original pHel12 plasmid of the recipient strain (black arrow, 0.4 kb). Lane 1, 1 kb ladder, lane 2, pHel12 used as template, lane 3 and 4, plasmid DNA from transconjugants 1 and 2 used as template.