Analysis of the PV94 cohesive ends.

<p>(A) AdeI, Bgll and SspI restriction patterns of PV94 phage DNA. Lane M, DNA size marker (λ DNA Eco130I), lanes (−), unheated phage DNA, lanes (+), phage DNA that had been heated before digestion. (B) Determination of protruding nucleotides. Chromatograms of run-off sequencing reactions using phage DNA as template and primers (PPNF and PPNR) binding close to the genomic ends. The 16 bp overhanging sequences are shaded and marked by arrows.