Baeyer-Villiger monooxygenases from microorganisms

The oxidation of bicyclo[3.2.0]hept-2-en-6-one and 7-endopropylbicyclo[3.2.0]hept-2-en-6-one was investigated using whole cells of Pseudomonas putida NCIMB 10007 and Xanthobacter autotrophicus NCIMB 10811. The bacteria demonstrated both regio- and enantioselective oxidation of the substrates. P. putida gave 'mirror image' products with both substrates when the products of these oxidations were compared with cells grown on the different enantiomers of camphor. The regio- and enantioselectivity of the oxidation of the substrates by X: autotrophicus were enhanced by the 7-endopropyl substitution of bicyclo[3.2.0]hept-2-en-6-on