Add to ORKGIncreasing substrate concentration during enzymatic protein hydrolysis results in a decrease in hydrolysis rate. To test if changes in the mechanism of hydrolysis also occur, the enzyme selectivity was determined. The selectivity is defined quantitatively as the relative rate of hydrolysis of each cleavage site in the protein. It was determined from the identification and quantification of the peptides present in the hydrolysates. Solutions of 0.1–10% (w/v) whey protein isolate (WPI) were hydrolyzed by Bacillus licheniformis protease at constant enzyme-to-substrate ratio. The cleavage sites were divided into five groups, from very high (>10%) to very low selectivity
The experimental maximum degree of hydrolysis (DHmax,exp) was typically lower than the theoretical m...
The objectives of this study were to hydrolyze whey proteins using a pancreatin and an Aspergillus o...
A whey protein concentrate (WPC) was produced from fresh whey by ultrafiltration (MW cut off 10 kDa)...
Increasing substrate concentration during enzymatic protein hydrolysis results in a decrease in hydr...
Increasing substrate concentration during enzymatic protein hydrolysis results in a decrease in hydr...
Enzymatic protein hydrolysis is typically described by the degree of hydrolysis and by the enzyme sp...
Enzyme selectivity is introduced as a quantitative parameter to describe the rate at which individua...
To understand the differences in peptide composition that result from variations in the conditions o...
Enzymatic hydrolysis of proteins increases the use of whey. In this study, a B. licheniformis protea...
During enzymatic hydrolysis of proteins, some cleavage sites will be accessible in the intact protei...
Changes in substrate concentration were shown to affect the experimental maximum degree of hydrolysi...
The extent of protein enzymatic hydrolysis is considered to be mostly determined by protease specifi...
To identify the parameters that affect enzymatic hydrolysis at high substrate concentrations, whey p...
The objective of this work was to identify the dominant aggregating peptides from a whey protein hyd...
Whey protein concentrate was enzymatically hydrolysed with the microbial proteases Alcalase® and Fla...
The experimental maximum degree of hydrolysis (DHmax,exp) was typically lower than the theoretical m...
The objectives of this study were to hydrolyze whey proteins using a pancreatin and an Aspergillus o...
A whey protein concentrate (WPC) was produced from fresh whey by ultrafiltration (MW cut off 10 kDa)...
Increasing substrate concentration during enzymatic protein hydrolysis results in a decrease in hydr...
Increasing substrate concentration during enzymatic protein hydrolysis results in a decrease in hydr...
Enzymatic protein hydrolysis is typically described by the degree of hydrolysis and by the enzyme sp...
Enzyme selectivity is introduced as a quantitative parameter to describe the rate at which individua...
To understand the differences in peptide composition that result from variations in the conditions o...
Enzymatic hydrolysis of proteins increases the use of whey. In this study, a B. licheniformis protea...
During enzymatic hydrolysis of proteins, some cleavage sites will be accessible in the intact protei...
Changes in substrate concentration were shown to affect the experimental maximum degree of hydrolysi...
The extent of protein enzymatic hydrolysis is considered to be mostly determined by protease specifi...
To identify the parameters that affect enzymatic hydrolysis at high substrate concentrations, whey p...
The objective of this work was to identify the dominant aggregating peptides from a whey protein hyd...
Whey protein concentrate was enzymatically hydrolysed with the microbial proteases Alcalase® and Fla...
The experimental maximum degree of hydrolysis (DHmax,exp) was typically lower than the theoretical m...
The objectives of this study were to hydrolyze whey proteins using a pancreatin and an Aspergillus o...
A whey protein concentrate (WPC) was produced from fresh whey by ultrafiltration (MW cut off 10 kDa)...