Partial Characterization Of A Whey Protein Concentrate And Its Enzyme Hydrolysates

A whey protein concentrate (WPC) was produced from fresh whey by ultrafiltration (MW cut off 10 kDa) and lyophilization. Enzymatic hydrolysis was performed with three enzyme systems: pancreatin (PA), protamex (PR) or alcalase 0.6L (AL) to produce hydrolysates with 20% degree of hydrolysis (DH). The peptide profiles of the hydrolysates were determined by high performance capillary electrophoresis (HPCE). The relationship between enzyme system and preferential protein substrate could be established. The alcalase hydrolysate (ALH) differed from the other two hydrolysates, and the enzyme showed the lowest specificity for β-lactoglobulin. Considering the protein content from WPC the pancreatin hydrolytic system was the most efficient leaving only 4.69% unhydrolyzed protein in the final hydrolysate (PAH). For 20% degree of hydrolysis alcalase left 7.98% unhydrolyzed protein, while protamex left 9.81% unhydrolyzed protein in the final hydrolysate.264327338Acton, G.H., The determination of lactose in cheese (1977) Austr. J. Dairy Technol., 9, pp. 111-114Adler-Nissen, J., (1986) Enzyme Hydrolysis of Food Proteins, p. 427. , Elsevier Applied Science Publishers, LondonHorwitz, W., (1990) Official Methods of Analysis, , Association of Official Analytical Chemists. 15 th Ed., Washington, D.CBligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can. J. Biochem. Physiol., 38, pp. 911-917Bounous, G., Gervais, F., Amer, V., Batist, G., Gold, P., The influence of dietary whey protein on tissue glutathione and the diseases of aging (1989) Clin. Invest. Med., 12, pp. 343-349Brink, W., The life extension protein: That fights disease and extends lifespan. Life extension report (1996) Life Extension Foundation, 1, pp. 21-28De Wit, J.N., Nutritional and functional characteristics of whey proteins in food products (1998) J. Dairy Sci., 81, pp. 597-608Frazier, R.A., Ames, J.M., Nursten, H.E., The development and application of capillary electrophoresis methods for food analysis (1999) Electrophoresis, 20, pp. 3156-3180Freitas, O., Padovan, G.J., Vilela, L., Santos, J.E., Dutra De Oliveira, J.E., Greene, L.J., Characterization of protein hydrolysates prepared for enteral nutrition (1993) J. Agric. Food Chem., 41, pp. 1432-1438Frokjaer, S., Use of hydrolysates for protein supplementation (1994) Food Technol., 10, pp. 86-88Gonzáles-Tello, P., Camacho, F., Jurado, E., Páez, M.P., Guadix, M., Enzymatic hydrolysis of whey proteins: II. Molecular weight range (1994) Biotechnol. Bioeng., 44, pp. 529-532Guzmán, G.J., Whey protein concentrate (80%) its importance in human nutrition (1995) Anais I Simpósio Latino Americano de Alimentos, pp. 1-6. , novembro, CampinasHeine, W.E., Klein, P.D., Reeds, P.J., The importance of α-lactoalbumin in infant nutrition: A critical review (1991) J. Nutr., 121, pp. 277-283Hettiarachchy, N.S., Ziegler, G.R., (1994) Protein Functionality in Food Systems, p. 519. , Marcel Dekker, New YorkJu, Z.Y., Otte, J., Madsen, J.S., Qvist, K.B., Effects of limited proteolysis on gelation and gel properties of whey protein isolate (1995) J. Dairy Sci., 78, pp. 2119-2128Lawson, M.A., Milk protein as food ingredients (1994) Food Technol., 48, p. 101Multilangi, W.A.M., Panyan, D., Kilara, A., Hydrolysates from proteolysis of heat-denatured whey proteins (1995) J. Food Sci., 60, pp. 1104-1109Otte, J.A.H.J., Kristiansen, K.R., Zakora, M., Qvist, K.B., Separations of individual whey proteins and measurement of α-lactoalbumin and β-lactoglobulin by capillary zone electrophoresis (1994) Neth. Milk Dairy J., 48, pp. 81-97Ovesen, L., Palatability and intake of two commercial liquid diets in patients with poor appetite (1991) Europ. J. Clin. Nutr., 45, pp. 273-275Schmidl, M.K., Taylor, S.L., Nordlee, J.A., Use of hydrolysate-based products in special medical diets (1994) Food Technol., 48, pp. 77-85Sgarbieri, V.C., (1996) Proteínas em alimentos protéicos: propriedades, degradações, modificações, p. 517. , Livraria Varela, São PauloStatistics for Windows (1995) Computer program Manual, , Statsoft In