A limiting factor in the efficacy of bioartificial liver (BAL) for the treatment of liver failure is the toxicity of the patients' serum to the hepatocytes in the device. This study investigates the interaction of liver cancer patient serum with primary and immortalised rat hepatocytes. Liver cancer serum increased the growth rate of immortalised hepatocytes, without affecting reduced glutathione levels. The activities of DT-diaphorase and pi glutathione-S-transferase (GST), enzymes associated with de-differentiation, were also increased. Exposure of primary hepatocytes to liver cancer serum resulted in a decrease in cytochrome P450 (CYP) content, and in P450 dependent metabolism of testosterone. Formation of 2-alpha- and 6-beta- hydroxy te...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...
A limiting factor in the efficacy of bioartificial liver (BAL) for the treatment of liver failure is...
A limiting factor in the efficacy of bioartificial liver (BAL) for the treatment of liver failure is...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
Abstract: The liver, the largest organ of the human body, is a multifunctional organ with various me...
OBJECTIVES: An extracorporeal bioartificial liver device must maintain viability and differentiated ...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...
A limiting factor in the efficacy of bioartificial liver (BAL) for the treatment of liver failure is...
A limiting factor in the efficacy of bioartificial liver (BAL) for the treatment of liver failure is...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
Abstract: The liver, the largest organ of the human body, is a multifunctional organ with various me...
OBJECTIVES: An extracorporeal bioartificial liver device must maintain viability and differentiated ...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...
Pathological disorders of the liver were shown to be associated with an impairment of hepatic drug m...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...
The preparation of hepatocytes using the two-stage perfusion technique usually involves the use of c...