Size-based fractionation of native milk fat globules by two-stage centrifugal separation

A two-stage centrifugal separation method, at various separation temperatures and feed rates, was employed to fractionate milk and cream on the basis of fat globule size. It involved a modified and a conventional centrifugal separation in first and second stages, respectively. In the first stage, two streams of milk: one rich in larger fat globules and another rich in smaller fat globules, were obtained by fractionation in a modified cream separator. In the second stage, the two streams from the first stage were each further fractionated in a conventional cream separator. Depending on the temperature and feed rate of the first stage, this double separation method was able to create streams with mean fat globule size (D [4, 3]) as small as 1.35\ua0μm and as large as 4.28\ua0μm without affecting the droplet integrity. The developed method has potential for size-based fractionation of native fat globules in industrial scale. Industrial relevance The developed method has potential for size based fractionation of native fat globules in industrial scale