A covalently immobilized antibody: The influence of the bond chemistry and support polymer on the uptake, stability and immunoreactivity

Attempts to scale up immunoaffinity separations have highlighted the limitations of the commonly employed CNBr-activated agarose gel supports. As an ai antibody covalently immobilized to styrene, nylon and acrylic acid using several different chemical methods. The level of uptake of antibody was found constant of the immobilized antibody averaged 1.5 × 10-6 M. This value was an order of magnitude lower than previously reported results for the support polymer used. The different covalent bonds were subjected to commonly encountered eluting agents. Most were stable in at least one solution cap bond in elutant was formed by glutaraldehyde-activated amino groups, followed by bonds formed through N-hydroxysuccinimide esters. Overall, the results in a highly stable bond without adversely affecting antibody immunological reactivity