We have investigated the complex formation between an immobilized monoclonal antibody and antigens that differ in size about 50-fold. As a model system, we used an iodinated progesterone derivative and a progesterone-horseradish peroxidase conjugate as tracer and a monoclonal antibody as binding protein. The antibody was immobilized by four different methods: physical adsorption, chemical binding, and binding via protein G in the absence or presence of a protective protein (gelatin). These investigations have shown that the performance of competitive immunoassays is determined by a combination of factors: (a) the relative size of the analyte and the tracer, (b) the antibody density on the solid matrix, (c) the method of immobilization of th...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...
Immunochromatographic assays are convenient analytical methods to measure analytes under non-laborat...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...
We describe optimal conditions for immobilization of two monoclonal antibodies to progesterone for s...
In an attempt to optimize immunosensors operating with an immobilized antibody as binding protein an...
Immunochemical reagents were characterized under carefully controlled laboratory conditions using co...
Immunological reagents were prepared and characterized for the development of analytical methodology...
We present a solid-phase immobilization method for radio-ligand assays, using an immunoadsorption co...
The discovery and characterization of oncofetal proteins have led to significant advances in early c...
We investigated the synthesis of progesterone-horseradish peroxidase (P-HRP) conjugates and products...
Antibodies to myoglobin were immobilized by covalent linkage to polyester film for use in a solid-ph...
Monoclonal antibodies against progesterone conjugated to carrier protein through substituent on the ...
Monoclonal antibodies against progesterone conjugated to carrier protein through substituent on the ...
Monoclonal antibodies against progesterone conjugated to carrier protein through substituent on the ...
Immunosensors for the detection of small analytes that use analyte-enzyme conjugates as signal gener...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...
Immunochromatographic assays are convenient analytical methods to measure analytes under non-laborat...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...
We describe optimal conditions for immobilization of two monoclonal antibodies to progesterone for s...
In an attempt to optimize immunosensors operating with an immobilized antibody as binding protein an...
Immunochemical reagents were characterized under carefully controlled laboratory conditions using co...
Immunological reagents were prepared and characterized for the development of analytical methodology...
We present a solid-phase immobilization method for radio-ligand assays, using an immunoadsorption co...
The discovery and characterization of oncofetal proteins have led to significant advances in early c...
We investigated the synthesis of progesterone-horseradish peroxidase (P-HRP) conjugates and products...
Antibodies to myoglobin were immobilized by covalent linkage to polyester film for use in a solid-ph...
Monoclonal antibodies against progesterone conjugated to carrier protein through substituent on the ...
Monoclonal antibodies against progesterone conjugated to carrier protein through substituent on the ...
Monoclonal antibodies against progesterone conjugated to carrier protein through substituent on the ...
Immunosensors for the detection of small analytes that use analyte-enzyme conjugates as signal gener...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...
Immunochromatographic assays are convenient analytical methods to measure analytes under non-laborat...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...