Delayed hypersensitivity to chemically induced tumors in mice and correlation with an in vitro test

Tumors induced in CBA mice by 3-methylcholanthrene were used to confer delayed hypersensitivity on other mice of the same strain. Two methods of sensitization were successful: 1) surgical removal of a transplanted growing tumor and 2) administration of a disrupted tumor vaccine with Freund's adjuvant. Living tumor cells prepared in various ways (but not disrupted cells or extracts) elicited typical delayed cutaneous reactions when injected into the foot pads of sensitized mice; these reactions were tumor specific. Hypersensitivity in vivo was correlated with specific susceptibility to the inhibition of peritoneal cell migration in vitro