Crystallization and preliminary crystallographic analysis of the signal recognition particle SRPΦ14-9 fusion protein

AbstractThe SRPΦ14-9 fusion protein, which can functionally replace the SRP9/14 heterodimer in the mammalian signal recognition particle (SRP), has been crystallized using the vapor diffusion method. Four different crystal forms were grown. SRPΦ14-9 form IV crystals belong to the space group P4122/P4322 with cell parameters a = b = 69.7 Å, c = 95.7 Å, α = β = γ = 90°. A complete data set to 2.8 Å resolution with an Rsym on intensities of 7.0% was collected on a single flashfrozen crystal