Structure of three acidic O-linked carbohydrate chains of porcine zona pellucida glycoproteins
- Hokke, Comelis H.
- Damm, Jan B.L.
- Kamerling, Johannis P.
- Vliegenthart, Johannes F.G.
DOIAbstract
AbstractStructural analysis by ID and 2D 1H NMR spectroscopy of three acidic O-linked oligosaccharide alditols, released from porcine zona pellucida glycoproteins by alkaline borohydride treatment, afforded the following structures: Galβl-4(6SO4-)GlcNAcβl-3Galβl-4GlcNAcβ1-3Galβ1-3GalNAc-ol Neu5Gcα2-3Gal/91 -4(6SO4-)GlcNAqβl -3Ga1βl -4GlcNAcβl -3Galβl -3GalNAc-ol Neu5Acα2-3Galβl -4(6SO4-)GlcNAcβl -3Galβl -4GlcNAcβl -3Galβl-3GalNAc-ol These oligosaccharides are the smallest compounds that contain the structural elements which are present in the acidic, high-molecular mass O-linked carbohydrate chains of porcine zona pellucida glycoproteins