Sulfated N-linked carbohydrate chains in porcine thyroglobulin
- Kamerling, Johannis P.
- Rijkse, Irma
- Maas, Augustinus A.M.
- van Kuik, J.Albert
- Vliegenthart, Johannes F.G.
DOIAbstract
AbstractN-linked carbohydrate chains of porcine thyroglobulin were released by the hydrazinolysis procedure. The resulting mixture of oligosaccharide-alditols was fractionated by high-voltage paper electrophoresis, the acidic fractions were further separated by high-performance liquid chromatography on Lichrosorb-NH2, and analyzed by 500-MHz 1H-NMR spectroscopy and, partially, by permethylation analysis. Of the acidic oligosaccharide-alditols, the following sulfated carbohydrate chains could be identified
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