The Frizzled Planar Cell Polarity signaling pathway controls Drosophila wing topography

AbstractThe Drosophila wing is a primary model system for studying the genetic control of epithelial Planar Cell Polarity (PCP). Each wing epithelial cell produces a distally pointing hair under the control of the Frizzled (Fz) PCP signaling pathway. Here, we show that Fz PCP signaling also controls the formation and orientation of ridges on the adult wing membrane. Ridge formation requires hexagonal cell packing, consistent with published data showing that Fz PCP signaling promotes hexagonal packing in developing wing epithelia. In contrast to hair polarity, ridge orientation differs across the wing and is primarily anteroposterior (A–P) in the anterior and proximodistal (P–D) in the posterior. We present evidence that A–P ridge specification is genetically distinct from P–D ridge organization and occurs later in wing development. We propose a two-phase model for PCP specification in the wing. P–D ridges are specified in an Early PCP Phase and both A–P ridges and distally pointing hairs in a Late PCP Phase. Our data suggest that isoforms of the Fz PCP pathway protein Prickle are differentially required for the two PCP Phases, with the Spiny-legs isoform primarily active in the Early PCP Phase and the Prickle isoform in the Late PCP Phase