The major acidic glycolipids from the kidney of the pacific salmon (Oncorhynchus keta): Characterization of a novel ganglioside, fucosyl-N-acetylgalactosaminyl-GM1

Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (XI) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methylation analysis, chemical and enzymatic degradation, reaction with monoclonal antibodies, LSIMS, and 'H-NMR spectroscopy, XI was deter-mined to be a monosialosyl ganglioside belonging to the ganglio-series with a unique FuCffl-3GalNAc linkage at the nonreducing terminal: Fucal-3GalNAc^l-3Galy91-3GalNAcy91-4[NeuAca2-3]Galy91-4Glcy91-lCer Analysis of the lipophilic moiety indicated predominance of 24:1 fatty acid in combination with sphingenine. X2 was found to have a glycon structure identical to XI. The ceramide of X2 consisted predominantly of saturated fatty acids (18:0 and 16:0). The tissue concentra-tions of XI and X2 in kidney were 3.7 and 2.8 nmol/g, respectively. Key words: fucoganglioside, 'H-NMR spectroscopy, LSIMS, kidney, Oncorhynchus keta, salmon. Glycolipids are the components of the animal plasma membrane (3, 4), and those in mammalian kidney have been studied with respect to renal functions (5-8). Quanti-tative estimation of renal sulfoglycolipids in various mammals indicated that the sulfoglycolipid content is a function of metabolic activities (9, 10) as well as the 1 This work was supported in part by a Grant-in-Aid for Co-operativ