Comparative investigation of glycosphingolipid composition of Japanese pilchard head

The distribution and composition of glycosphingolipid in the head that of Japanese pilchard were studied. The head (wet weight=81.4g) was removed from six Japanese pilchards. The total glycosphingolipid of the head was extracted with the procedure of Svennerholm and divided into four fractions. (neutral glycolipid: N. L., sulfatide: CSE, monosialoganglioside: GM and polysialoganglioside: ≧GD) The results of our experiment are as follows: 1) The head included N. L., CSE and ganglioside of low concentration compared with various animal brains. 2) The head contained monosialoganglioside as the main component of ganglioside; where the fish brain contained polysialoganglioside predominantly. Monosialoganglioside, which mainly consisted of GM3 and GM2, occupied 64% of ganglioside. 3) Three components derived from CMH were the principal constituents of neutral glycolipid in the head.6KJ00004311118報文Originaldepartmental bulletin pape