Add to ORKGTransfer RNA (tRNA) is the adaptor molecule that translates a nucleic acid sequence from the genome into a protein sequence. tRNAs are transcribed as precursor molecules (pre-tRNAs) that have extra sequence on both the 5’ and 3’ ends that must be removed before the tRNA can be functional in translation. Ribonuclease Ps (RNase Ps) are metal-dependent endonucleases responsible for the 5’ end maturation of pre-tRNAs. All domains of life encode an RNA-dependent RNase P, with a conserved RNA component that is fully catalytic in Bacteria. Eukaryotes may also encode protein-only RNase Ps (PRORPs). The PRORPs from the land plant Arabidopsis thaliana are the only RNase Ps responsible for 5’ end maturation of pre-tRNAs in all three tRNA-encoding cel...
