Synthesis of Novel Nicotinamide Adenine Dinucleotide (NAD) Analogs and Their Coenzyme Activities

Four methods were tested to synthesize nicotinamide adenine dinucleotide (NAD) analogs, and 5 NAD analogs were prepared. Assays of these NAD analogs indicated that malic enzyme and alcohol dehydrogenase had about 13%similar to 30% enzymatic activity in the presence of analog 3b or 3d, whereas significantly lower activities were observed in the presence of analog 3a, 3c or 3e