超沈澱せるActomyosin溶解に対する低温Pyrophosphate及びSalyrganの影響

Spicer & Gergely obstrved that superprecipited actomyosin (AM) can be dissolved under a certain condition. From this observation, Szent-Gyorgyi assumed that AM dissociates in aecordonce with the decrease temperature. The authors have empasized that muscular contraction is a specific process utili-zing the splitting energy of ATP but that relaxation is a non-specific to ATP. Therefore, in the present paper the results observed by Spicer were reexamined. The primary object of the present studies is to determie whether superprecipitated AM can be dissolved by pyrophosphate or salyrgan which are known to bring about relaxation or derigor of the glycerinated muscle fiber. The results are as follows: ・ I) Superprecipitation did not occur at 0ーC, but occurred when the solution was warmed to 25ーC. The .superprecipitate, formed at 25ーC, was dissolved by cooling at 0ーC in the presence of ATP. 2) Superprecipitate was dissolved by addition of pyrophosphate at the rate of 5X10-3 M or over, and it was even dissolved by an addition of 10-3M pyrophosphate in the presence of ATP. 3) Superpercipitate was not dissolved even in the presence of 5X10-4?10-2 salyrgan, but it was dissolved by an addition of salyrgan of over 5X10-3M in the presence of ATP