Ectromelia virus 感染孵化鷄卵漿尿膜の組織化学的および生化学的研究 第2篇 生化学的研究

In the preceding report, the histological and histochemical pattern of the chorioallantoic membranes infected in vivo with ectromelia virus were presented. The present paper deals with the metabolic changes in the membranes infected in vivo or in vitro with the virus, as biochemically examined. 1. Metabolic changes developing in the infected membranes were characterized by an increase in anaerobic glycolysis, lactic acid, pyruvic acid and in inorganic phoshate, by a decrease in ATP-ADP phosphate and by an alteration in aerobic oxydation. 2. Almost no difference in the rate of endogenous oxygen uptake between the infected and the control membranes was observed. However, the decrease in cytochrome c, of cytochrome oxydase activity and the increase of several kinds of dehydrogenase activities involved in the Krebs cycle were noticed. "Hydroquinone oxydase" activity, an enzyme regarded as one of flavoproteins, did not decrease in the infected membranes. 3. Respiratory quotient in the infected membranes was higher than in the controls, the framer indicdting a value of approximately 1.1, while the latter showed 0.8. 4. The rate of inhibition of endogenous oxygen uptake for malonate was 28% in the infected membranes, while standing at 46% in the controls. 5. No difference was observed in the oxygen uptake between the membranes cultured in the Warburg flask with or without the presence of the virus. The virus, however, decreased during the first 24 hours and thereafter increasing gradually, reached a titer similar to that of the inoculum 48 hours after inoculation. 6. As histochemically and biochemically observed, the metabolic pattern developed in the virus-infected chorioallantoic membranes are considered to be characterized by an increase in the function of the host tissues which results in proliferation of the cells of all the three layers of the membranes as in well as multiplication of the virus. In so far as examined, the specificity of the host tissues may lie in the mechanism by which energy for the tissues is produced