Ectromelia Virus感染マウス肝の組織化学的及び生化学的研究 第1篇 組織学的及び組織化学的研究

The present study was undertaken in order to obtain some clues as regards the appreciation of virus lesions developed in the liver of mice inoculated with ectromelia virus. The livers of infected mice were examined for their virus content and their histological and histochemical characters of lesions. Clinical symptoms were manifested 3 to 4 days after an inoculation of a larger amount of virus. The virus appeared in the liver for the first time, 12 hours after inoculation, then steadily increased in amount, with the virus titer reaching 3.2 x 10 8 LD50, in moribund mice in the final stages. The lesions in the liver of moribund mice were characteristic of the focal necrosis surrounded with the hypertrophic area of liver cells and accompanied with scanty cellular infiltrations. When routinely examined, almost no remarkable changes were observed in the liver cells during the incubation in spite of a steady increase of virus. Applications of some histochemical methods, however, led to the establishent of certain available information regarding the pathogenesis of virus lesions. The methylgreen-pyronine staining showed significant strengthening of pyronine staining in the cytoplasms and in the nucleoli of the liver cells in the incubation period. An acceleration of activity of alkaline-phosphatase also was observed by the Gomori method. From these histochemical findings it is suggested that the function of the liver cells might possibly be converted to a protein synthesis coupled with virus reproduction