(17(4):65-74)PHYSIOLOGICAL STUDY OF TRUFFLE DISEASE ON CULTIVATED MUSHROOM

1.腦菌病病原菌對氨態氮之生理利用，程度甚低，但稍有能利用硝酸態氮之趨勢。 2.鹽類如NaCl及（NH4）2SO4濃度4N以上處理腦菌病病原菌子囊果3小時以上，可因其滲透壓之作用抑制其生長。 3.60℃乾濕熱處理洋菇各種病原菌除Mucor, Thielavia sp.及Trichoderma sp.外，其餘處理2小時以上，即達其致死程度。 4.Trichoderma sp.分泌之Toxin證實對洋菇腦菌病病原菌有促進生長與發育之趨勢，但在相同情況下，對洋菇則否。 5.洋菇以547品系對Trichoderma sp.之抗性最強次為542、543品系541最弱，培養基中含20%以上之Trichoderma sp.汁液即顯著影響洋藷菌絲生長。 Ammonium nitrogen was failure to be utilized as sources of nitrogen by pathogen of truffle disease (Diehliomyces microsporus). but it was generally used to support the growth of cultivated mushroom (Agaricus bisporus). Only nitrate nitrogen could be used by Diehliomyces microsporus. That was a significant different about nitrogen nutrition between cultivated mushroom and truffle disease. The addition of the solution of salts as NaCl and (NH4)2SO4 at the concentration above 4N to ascocarps. of Diehliomyces microsporus, could control the growth of the fungus. Most mushroom diseases pathogens were killed with heat(140°F) under moist and dry condi-tions. Exceptions are Thielavia sp.,Mucor sp. and Trichoderma sp. A metabolic product as toxin of Trichoderma sp. had been testified that it could promote the development of the causal organism, but under the same conditions it was ineffective to growth of cultivated mushroom furthermore it decresed the yield. In regard with the resistance of several varieties of cultivated mushroom to Trichoderma sp., this experiment was proved that 547 (303) variety was the best one. 542 and 543 variety came next and 541 variety came after that. If the medium contained above 20% of the filtrate Trichoderma sp., it had great influented on growth of hyphae of cultivated mushroom